《Table 1–Soybean accessions used in the study.》

《Table 1–Soybean accessions used in the study.》   提示:宽带有限、当前游客访问压缩模式
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《Development and validation of InDel markers for identification of QTL underlying flowering time in soybean》


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Fifty six accessions,including 29 from three recent research papers and 27 from this study,were used for In Del polymorphism validation(Table 1).Young leaves from 27accessions were collected three weeks after planting in growth chambers and separately quick-frozen in liquid nitrogen.Total DNA was extracted by the improved cetyltrimethylammonium bromide(CTAB)method[36].A sequencing library was constructed with at least 6μg of genomic DNA following the manufacturer's instructions(Illumina Inc.,San Diego,CA).Paired-end sequencing libraries with an insert size of approximately 500 bp were sequenced on an Illumina Hi Seq 2000 sequencer.