《Table 2 Ranking of the 10 housekeeping genes using four different algorithms1)》

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《Selection of reference genes for RT-qPCR analysis of Phenacoccus solenopsis(Hemiptera: Pseudococcidae) sex-dimorphic development》

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1) SV，standard value；SD，standard deviation.

Ten candidate genes，EF1-β，EF1-δ，RP-L32，RP-L40，RP-18S，RP-28S，STX16，TUB-α，TBP and UB-E2 were selected for this study，and used in performing RT-qPCR.All genes were publicly available and had been used as reference genes in insects（Li et al.2013；Fu et al.2013；Yang et al.2014；Zheng et al.2014；Vea et al.2016）.The sequences of these genes were acquired from P.solenopsis transcriptome data（unpublished data）.The primers were designed using NCBI Primer-BLAST，and primer sequences are listed in Table 1.