《Table 3Sperm deformity in mice caused by B.thuringiensis 29.118》

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《传统发酵食品臭鳜鱼中分离的苏云金芽孢杆菌(Bacillus thuringiensis 29.118)的毒理学评价（英文）》

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In this study，sperm abnormality test was used to investigate that whether B.thuringiensis 29.118 had a reproductive toxicity to the male mice.The head of sperm changed into a red color by dyeing with a concentration of 2%eosin.The result showed different types of sperm deformity including normal sperm，non-hook sperm，chubby head sperm，banana-shaped sperm（Fig.2）were distinguished according to their shapes.The statistic results of malformation types and numbers were shown in Table 3.The percentages of malformation of the control，low-dose，medium-dose，high-dose，and the positive respectively were 6.9，7.2，8.5，7.9and 32.3.The sperm deformity rate was significantly different between positive control and negative control（P<0.01）.There was no significant difference between the treatment and the control groups（P>0.05）.Therefore，the tested B.thuringiensis did not lead to the deformity of sperm under the given conditions at the level of statistics.