《Table 5 Comparison of substrate selectivity between the proposed method and DNS method (n=3)》

《Table 5 Comparison of substrate selectivity between the proposed method and DNS method (n=3)》   提示:宽带有限、当前游客访问压缩模式
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《一种基于血糖仪的简便且微量的β-葡萄糖苷酶活性检测方法(英文)》


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The DNS assay is most popular for the detection ofβ-glucosidase activity.However,it requires the reaction of sugar and DNS.To simplify the experimental procedure and simultaneously reduce the consumption of both sample and reagent,differentreaction mixture volumes(40 to 2000μL)were studied.As shown in Table 6,the activity of crude almondβ-glucosidase samples was similar at different volumes.The smallest volume of 40μL was sufficient to detectβ-glucosidase activity using the proposed assay.In addition,the proposed method was free from requiring a chromogenic reaction.Thus,the proposed method was shown to be a simpler,lower-cost,and more efficient method than the DNS assay.