《Table 9 Statistical comparison of the proposed method with the pNPG assay (n=3)》

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《一种基于血糖仪的简便且微量的β-葡萄糖苷酶活性检测方法（英文）》

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a The values in parenthesis are the corresponding theoretical values of t and F at P=0.05

Traditional analytical methods for detectingβ-glucosidase activity include the dinitrosalicylic acid（DNS）assay（Hu et al.，2013）and p-nitrophenyl-β-D-glucopyranoside（pNPG）spectrophotometric assay（Rather et al.，2010；Strahsburger et al.，2017）.The mechanisms of these two methods are based on the liberation of monosaccharides and subsequent formation of colored or fluorescent substances，as shown in Table 1.Although they are commonly adopted in many experiments，both methods are time-and reagentconsuming，complex in operation，and require expensive instruments and trained personnel，which are not feasible for point-of-care testing（POCT）and impede their broad application in resource-limited regions.