《Table 1 qRT-PCR primers of MdCER family》

《Table 1 qRT-PCR primers of MdCER family》   提示:宽带有限、当前游客访问压缩模式
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《"The Characterization, Authentication, and Gene Expression Pattern of the MdCER Family in Malus domestica"》


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Total RNA was extracted using an RNA plant reagent(Tiangen,China).The RNA(1–7μg)was reverse-transcribed using the Prime Script first-strand cDNA synthesis kit(Ta Ka Ra,China).Quantitative real-time(q RT)PCR was used to detect the expression level of the Md CER family in different apple organs and in response to ABA and PEG.Md Actin(GenBank accession number:CN938024)was selected as an internal control gene.All of the primers are shown in Table 1.The results were based on the average of three parallel experiments.