《Tab.1 Primers used in the test》

《Tab.1 Primers used in the test》   提示:宽带有限、当前游客访问压缩模式
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《Screening of Host Proteins Interacting with Porcine Epidemic Diarrhea Virus (PEDV) N Protein by Yeast Two-hybrid System》


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Primers were designed according to the complete sequence of N,LGA LS3 and SNRPG proteins using Primer5.0 software.Following the method of IN-Fusion clone connection kit,viscous terminal sequence of Bam HⅠdigestion site were introduced on both ends of the primer;the 5’end of the primer contained15 base sequences equal to the terminal ofvectorspGBKT7,pGADT7and p3xFLAGCMV-7.1,and the 3’end contained the specific base sequences complementary to target gene fragments(Tab.1).The primers were synthesized by Sangon Biotech(Shanghai)Co.,Ltd..