《Tab.1 Primers used in the test》
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《Screening of Host Proteins Interacting with Porcine Epidemic Diarrhea Virus (PEDV) N Protein by Yeast Two-hybrid System》
Primers were designed according to the complete sequence of N,LGA LS3 and SNRPG proteins using Primer5.0 software.Following the method of IN-Fusion clone connection kit,viscous terminal sequence of Bam HⅠdigestion site were introduced on both ends of the primer;the 5’end of the primer contained15 base sequences equal to the terminal ofvectorspGBKT7,pGADT7and p3xFLAGCMV-7.1,and the 3’end contained the specific base sequences complementary to target gene fragments(Tab.1).The primers were synthesized by Sangon Biotech(Shanghai)Co.,Ltd..
图表编号 | XD00183483800 严禁用于非法目的 |
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绘制时间 | 2018.08.01 |
作者 | Wang Zhongze、Qin Cuili、Kong Ning、Zuo Yewen、Wang Meng、Zheng Hao、Tong Wu、Li Liwei、Yu Hai、Li Zhili、Shan Tongling、Tong Guangzhi、Li Xue |
绘制单位 | Henan University of Science and Technology、Henan University of Science and Technology、Shanghai Institute of Veterinary Medicine, Chinese Academy of Agricultural Sciences、Shanghai Institute of Veterinary Medicine, Chinese Academy of Agricultural Sciences、S |
更多格式 | 高清、无水印(增值服务) |