《Table 2 Variation of glucosinolate contents at different growing stages of turnip》

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《Glucosinolate Variation in Turnip (Brassica rapa L.) at Different Growing Stages》

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μmol/g DW

HPLC analysis condition:Waters C18 chromatographic column，3.9 mm×150 mm，4μm；detection wavelength:229 nm；column temperature:25℃；sample volume:10μl；flow rate of mobile phase:1.0 ml/min.The gradient elution program is shown in Table 1.The mobile phase was prepared by dissolving 0.5 g of tetramethylammonium chloride（TMACl）in 1 L of water（18.2 MΩ·cm），mixing and vacuum filtration.The mobile phase B was prepared by dissolving 0.5 g of tetramethylammonium chloride（TMACl）in 0.8 L of water（18.2MΩ·cm），adding 200 ml of chromatographically pure acetonitrile，mixing and vacuum filtration.TRO was used as the internal standard，and glucosinolate components were quantitatively determined according to the retention time and peak area.The contents of glucosinolates（μmol/g DW）were calculated according to the internal standard and response factor.