《Table 4Results from metabolic pathway analysis with Metabo Analyst 3.0.a》

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《Inhibition of metabolic disorders in vivo and in vitro by main constituent of Coreopsis tinctoria》

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aTotal is total number of compounds in pathway；Hits are actually matched number from user uploaded data；Raw p is original p-value calculated from enrichment analysis；FDR（False Discovery Rate）is also from enrichment analysis；Impact is pathway impact value

Cells treated with high glucose or marein were collected to isolate the total RNA.Total RNA was reverse transcribed，and PEPCK，G6Pase，FH，MDH2，SDHA，SDHB，IDH2，ACO2，CS，and glyceraldehyde-3-phosphate dehydrogenase（GAPDH）gene expression was measured by q PCR using an i Cycler thermocycler（Bio Rad，Hercules，CA）as previously described（Mac Donald et al.，2013）.The primers for these genes were listed in Table 1.All samples were analyzed in triplicate，and the gene expression levels were normalized to the control human GAPDH values.The relative expression of the treated samples was normalized to that of the untreated cells according to the Ct analysis.