《Table 1 Comparison of lateral flow strip test and conventional method (ELISA) for RAC detection in

《Table 1 Comparison of lateral flow strip test and conventional method (ELISA) for RAC detection in   提示:宽带有限、当前游客访问压缩模式
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《一种新型莱克多巴胺-蛋白质载体结合技术在侧向流试纸条检测动物饲料中莱克多巴胺含量中的应用(英文)》


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The values of RAC concentrations are expressed as mean±SD

To test the practical applicability of the strip test,RAC spiked into animal feed at 0.10,0.25,and0.50 ng/g was measured to calculate for percent recovery and relative standard derivation(RSD)percentage using an independent strip test on the same day(intra-assay)and on three different days(interassay),and the values obtained were compared with those obtained using the conventional method(ELISA)as summarized in Table 1.The intra-assay(n=6)recovery percentage was in the range of 96.9–100.2with RSD of 2.5%–2.8%,and the inter-assay(n=3)recovery percentage was 96.4–103.7 with an RSD of 3.1%–3.6%.The results demonstrated smaller RSD percentages for both intra-and inter-assays,demonstrating the precision of the developed method.To compare the results obtained using the strip test with those obtained using ELISA,a paired t-test at a95%confidence interval was used for animal feed analysis.The calculated t-value obtained by the strip test was lower than the critical t-value,indicating no significant difference between the strip test and ELISA.Therefore,the proposed strip test,with high reproducibility and precision,could have strong potential as an alternative device for the early screening of RAC in animal feeds.