《Table 3 Mortality rate of diff erent stocking density during the experiment》

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《Effects of stocking density on the growth and immunity of Atlantic salmon salmo salar reared in recirculating aquaculture system(RAS)》

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Data presented as mean±SE.Diff erent letters indicate statistical signifi cance at P<0.05.

About 5 872 of Atlantic salmon with an average body mass of 1 413.1±52.3 g were purchased from Shandong Oriental Ocean Sci-Tech Co.，Ltd.Shandong Province，China.The fi sh were distributed to the 5 experimental RAS tanks（6 m×6 m×3 m）randomly.The initial density of each tank is，A:24 kg/m 3；B:21 kg/m 3；C:15 kg/m 3；D:9 kg/m 3 and E:6 kg/m 3.Experimental trials were conducted for220 days.During the experimental period，water temperature was maintained at 15.0±0.7°C，pH 7.2–7.5，total ammonia-nitrogen<0.25 mg/L，salinity between 28.0±0.5.Water was supplied to each tank at a rate of approximately 50 L/min with liquid oxygen to maintain dissolved oxygen between 130±10%saturation in the tank.Illumination was continuous and provided by one fl uorescent tube light per tank with light intensity of 60±10 lux on the surface of water.Fish were fed with a commercial salmon diet（Skretting，Norway）containing 42%protein and 22%fat at satiation level three times a day by fi sh automatic feeder（at about 6:00 AM，14:00 PM and 20:00 PM）during the period of light-manipulation.The stocking density variations were showed in Table 1.All procedures described in this study were reviewed and approved by the ethical committee of Institute of Oceanology，Chinese Academy of Sciences.