《Table 2–PHA accumulation assays using an enriched MMC fed with acetate and brewery wastewater.》

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《Comparative study of polyhydroxyalkanoates production from acidified and anaerobically treated brewery wastewater using enriched mixed microbial culture》

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Brewery wastewater after anaerobic treatment for methane production still contains a considerable concentration of organic carbon that has to be further reduced prior to discharge into the receiving water bodies（Table 1）.Therefore，the feasibility of using wastewater containing a low concentration of VFA for PHA production by the enriched MMC was evaluated.The wastewater was fed by pulse addition（six pulses of 177 mg C/L TOC）controlled by the DO concentration.Fig.6 shows the TOC，PHA and DO concentration profiles in the accumulation reactor fed with anaerobically treated brewery wastewater.The maximum PHA storage capacity achieved was 43.7%CDW，which consisted of PHB（29.5%CDW）and a smaller amount of PHV（14.2%CDW），and the maximum PHA storage yield was 0.49 Cmol/Cmol.The propionic acid in the wastewater contributed to the PHV content of PHA（Dionisi et al.，2004）.