《Table 2 System of reverse transcription》

《Table 2 System of reverse transcription》   提示:宽带有限、当前游客访问压缩模式
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《"Expression of TAC1 and Its Receptors TACR1,TACR2,TACR3 Genes in Different Goat Tissues Under Different Photoperiods"》


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The reaction system of the real-time fluorescent quantitative PCR had a total volume of 20μl,as shown in Table 3.The PCR was started with pre-denaturation at 95℃for 5 min,followed by 40 cycles of denaturation at 95℃for 10 s and annealing at 60℃for 30 s.After the reaction,the melting curve was analyzed.