《Table 3–The effect of CS loading methods;immersion (IM) , freeze-drying (FD1) and double freeze-dry

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《Novel technique of insulin loading into porous carriers for oral delivery》

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Different concentrations of CS were tested to choose the optimum amount of CS to coat the particles without inducing aggregation or reducing drug loading efficiency.When studying the effect of various concentrations of both low and high Mwt CS on insulin LC%with PLGA MPs（Table 2），it was found that insulin LC%significantly increased（P<0.05）as the concentration of CS increased.It reached a LC%value of 20%±0.9%at CS concentration 5 mg/ml，after which this finding was reversed and the LC%decreased to 6.9%±0.9%and 3.6%±0.3%at CS concentration of 10 and 15 mg/ml respectively.