《Table 1.All possible single-nucleotide mismatches of target AAVS1.》

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《单核苷酸错配对于Cpf1特异性的影响（英文）》

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The original pcrRNA was cut by endonuclease BsmBI and inserted a sequence of target site，thus generating a target-specific pcrRNA.Similar process was performed in pTarget with endonuclease BglII and XholI to produce a plasmid carrying PAM（TTTN）and target sequence.In order to simulate every possible single-nucleotide mismatch，we carried out all the base mutation with full PAM and spacer sequence.For example，the entire list of mismatched sequence of AAVS1 was shown in Table 1.