《Table 1 Fatty acid profi les (expressed as%TFA) under N-replete (N+) or N-deplete (N-) conditions a

《Table 1 Fatty acid profi les (expressed as%TFA) under N-replete (N+) or N-deplete (N-) conditions a   提示:宽带有限、当前游客访问压缩模式
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《"Metabolic and physiological regulation of Chlorella sp.(Trebouxiophyceae, Chlorophyta) under nitrogen deprivation"》


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Data are presented as mean±SD(n=3).Signifi cant diff erence between N+and N-conditions are indicated by asterisks(*P<0.05;**P<0.01).

To assess the metabolic diff erence at various time points,PCA and OPLS-DA model were used to discriminate the two sample groups(N+and N-)using the fi rst two principal components.Distinct clustering was observed in the data of day 2 and day 4(Fig.4).The parameters of R 2 X,R 2 Y and Q 2 revealed high discriminative and predictive stability of the model.From over a thousand features aligned in XCMS online,only 59 were selected and annotated using METLIN database based on statistical analysis(P<0.05,fold change>1.5)and their biological roles in Chlorella sp.Overall,the metabolites belonged to several classes,namely amino acids,lipids and fatty acids,nucleic acids,organic acid,phytochemical compounds,vitamins and cofactors.The heat map in Table 2 shows the fold change between N+and N-condition at day 1,2 and 4.The annotated metabolites were classifi ed using the KEGG BRITE mapping tool.