《Table 1–Conserved motifs identified in Glu-1 promoters.》

  提示：宽带有限、当前游客访问压缩模式

本系列图表出处文件名：随高清版一同展现

《Dissecting conserved cis-regulatory modules of Glu-1 promoters which confer the highly active endosperm-specific expression via stable wheat transformation》

1. 获取 高清版本忘记账户？点击这里登录

1. 下载图表忘记账户？点击这里登录

The Glu-1Dx2 promoter was selected for vector construction and functional validation.To assess the function of CCRMs，progressive 5′deletion fragments were generated from Glu-1Dx2 promoter and fused to the GUS reporter gene.Five fragments corresponding to 100-bp（core region），208-bp（core region plus CCRM1-1），300-bp（core region plus CCRM1），650-bp（core region plus CCRM1 and CCRM2），and 950-bp upstream（core region plus CCRM1，CCRM2，and CCRM3）of the start codon were amplified from Fielder genomic DNA and inserted into plant expression vector p Ubi-GUS to create p GUS100，p GUS208，p GUS300，p GUS650，and p GUS950，respectively.These constructs were introduced into Fielder by Agrobacterium tumefaciens mediated transformation using a licensed procedure（http://www.jti.co.jp/biotech/en/plantbiotech/index.html）.Transgenic lines were grown in the greenhouse at 20°C and 16/8-h photoperiod with supplementary lighting provided by high-pressure sodium vapor lamps（Powertone SON-T AGRO 400W；Philips Electronic UK）.Positive transgenic lines were identified by PCR with promoterspecific forward and common GUS-specific reverse primers.Primer sequences for vector construction and transgenic lines screening were shown in Table S1.