《Table 3 Results of clean reads mapping to Glymacine max genome》

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《Comparative Transcriptome Profiling of Glycine soja Roots Under Salinity and Alkalinity Stresses Using RNA-seq》

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The total RNA isolated from the root samples were used for RNA-seq analysis.RNA was reversetranscribed to c DNA by using Super Script TMⅢReverse Transcriptase kit（Invitrogen，Cal，USA）.The quantitative real-time PCR（q RT-PCR）was performed with each c DNA template using SYBR Premix ExTaq™；ⅡMix（Ta Ka Ra，Shiga，Japan）using an ABI7500 sequence detection system（Applied Biosystems，Carlsbad，CA）.GAPDH（accession#DQ355800）was used to normalize all the estimated values.The primers for q RT-PCR for 14 DEGs under salt and alkali stresses were designed using Primer Premier 5.0（http://www.premierbiosoft.com/primerdesign/）software and listed in Table 1a and Table 1b.The primers for q RT-PCR of eight MYB TFs under salt and alkali stresses are listed in Table 2a and Table 2b.Relative expression levels of DEGs were calculated using the2-ΔΔCT method.Three independent biological replicates were used for q RT-PCR in each of the three technical replicates.