《Table 1–Details of plant materials used.》

《Table 1–Details of plant materials used.》   提示:宽带有限、当前游客访问压缩模式
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《"Identification, development, and application of cross-species intron-spanning markers in lentil(Lens culinaris Medik.)"》


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Thirty-two Lens genotypes were used for genotyping with 105primers.A diverse panel of thirty-two Lens genotypes consisting of L.culinaris released cultivars,advanced breeding lines,parents of mapping populations,and genotypes of L.ervoides and L.culinaris subsp.orientalis was tested to identify polymorphic markers(Table 1).DNA samples were extracted from individual plant leaf tissue when seedlings were two weeks old using the cetyltrimethylammonium bromide(CTAB)procedure[21].The DNA concentrations of the extracted samples were recorded and were compared with after corresponding concentration withλDNA.The extracted DNA samples were diluted to a uniform concentration of 20μgμL-1for PCR amplification.