《Table 1 Clinical characteristics of patients with ileal Crohn’s disease》

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《Multiplex gene expression profile in inflamed mucosa of patients with Crohn's disease ileal localization: A pilot study》

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A panel of oligonucleotide capture probes was covalently linked to carboxylated fluorescently encoded beads（Luminex，Bio-Rad，MA，United States）.Each probe has a unique sequence of 15 bases.Each sample lysate diluted at 1:1 and 1:2 was mixed with the pooled capture beads in a round-bottom assay well and hybridized for 16 h at 54°C（final volume in each well was 100μL）.The assay mixture was moved to a MultiScreen?Filter Plate（Millipore，Billerica，MA，United States）and unbound material was filter-washed from the wells by rinsing 3 times with wash buffer.The plate was hybridized with 100μL/well of bDNA amplifier in Amplifier Diluent（Panomics，CA，United States）at 54°C for 1 h.After the plate was filter-washed twice with wash buffer and incubated at 50°C for 1 h with 100μL/well of 5’-dT（Biotin）-conjugated label probe（Panomics，CA，United States）diluted in Label Probe Diluent（Panomics，CA，United States）.After 2 washes，streptavidin-conjugated R-phycoerythrin diluted in SA-PE diluent（20 mmol/L Tris-HCl，400 mmol/L lithium chloride，1 mL/L Tween 20，1 mL/L bovine serum albumin，and 5 mL/L Micr-O-protect）was added and the plate was shaken and incubated at room temperature for30 min.We washed the beads to remove unbound SA-PE and then evaluated them with Bio-Plex?200 system（Bio-Rad，MA，United States）.The SA-PE fluorescence measured from each bead was proportional to the number of mRNA transcripts captured by the beads.Expression of target-specific RNA molecules was calculated as the mean values from triplicate cultures and normalized against Actin gene（high expression housekeeping gene）.