《Table 1Qualitative and quantitative variability in semi-polar and non-polar metabolites among diffe

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《"Phytochemical investigation, antioxidant and wound healing activities of Citrullus colocynthis (bitter apple)"》

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Mean values依SD of relative peak percentage area；*=quantified by HPLC（mean values依SD mg/g of dry weight）；ND=not detected；a，b，c，d，e，f，g，h，i，denote statistical significance P<0.05 i.e.a=Root vs Stem；b=Root vs Leaves；c=Root vs Fruit pulp；d=Root vs

Non-targeted metabolite profiling of hexane and methanolic extracts of all the five plant parts was carried out using GC-MS with five replicates of every sample.GC-MS based metabolite profiling of hexane extracts identified thirty chemically different metabolites comprising hydrocarbons，alkyl carboxylic acids，fatty acids，fatty alcohols，phenol，triterpene，vitamin and sterols（Figure2）.One-way ANOVA indicated that concentration of metabolites varied significantly among various parts of C.colocynthis（Table 1）.Linoleic and palmitic acid were detected as the major metabolites of hexane extracts in seeds.Percent peak area of palmitic acid ranged from（11.43±0.92）to（27.55±1.79），the lowest being in leaves and highest in seeds.Linoleic acid content ranged from（4.63±0.28）%in leaves to（33.92±1.65）%in seed with an average of（13.86±0.97）%in fruit pulp，respectively.The concentration of 2，4-di-tert butyl phenol ranged from（0.44±0.03）%to（3.20±0.23）%，which was lowest in leaves and highest in fruit pulp（Table 1）.