《Table 4Contents of saponinsin different parts of P.notoginseng and P.vietnamensis var.fuscidicus (m

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《Identification and quality analysis of Panax notoginseng and Panax vietnamensis var. fuscidicus through integrated DNA barcoding and HPLC》

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“-”represents lack of related information.*represents significant between same part of P.notoginseng and P.vietnamensis var.fuscidicus at P<0.05.

Codon Code Aligner V3.7.1（Codon Code Co.，USA）was performed to assemble raw data，delete low-quality regions，and remove 5.8 S and 28 S sections at both ends of the sequences.MEGA6.0 was employed for the final ITS2 sequence characteristic analysis.Sequence lengths，G+C content，and genetic distances were calculated.All of the samples and sequences from database（Table 1）were selected to construct a neighbor-joining tree（NJ tree）.Bootstrap tests were conducted using 1000 replicates to estimate the identification efficacy of phylogenetic relationships.