《Table 5 Results and analysis of orthogonal test》

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《Extraction Process and Content Determination of Total Flavonoids in Ginkgo( Ginkgo biloba L. ) Leaves》

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Standard curve y=0.0721x+0.001 1，R2=0.999 9；percentage content（%）=C×10×50/m×100%，wherein C is the concentration，m is the weight of ginkgo leaves.

At first，0.50，1.00，1.50，2.00，2.50 and 3.00 ml of quercetin standard solution were accurately pipetted into 10 ml volumetric flasks，respectively，followed by diluting to constant volume with methanol and shaking uniformly.The absorbance values of the obtained solutions were determined at 259.5 nm with methanol as a blank reference solution.A standard curve was drawn with the absorbance as the ordinate and the concentration as the abscissa.The concentration and absorbance values are shown in Table 1.The mass concentration of quercetin X（μg/ml）was in good linear relation with the absorbance Y in the range of6.50-39.0μg/ml.The linear regression equation of quercetin concentration X（μg/ml）and absorbance Y was Y=0.034 4X-0.020 1，R2=0.999 9.The linear relation was good.