《Table 12 Stability test of the test solution》

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《Extraction Process and Content Determination of Total Flavonoids in Ginkgo( Ginkgo biloba L. ) Leaves》

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At first，1.00 ml of the test solution was accurately pipetted into a 10 ml volumetric flask，and added with methanol to constant volume，followed by shaking uniformly.The absorbance at the maximum absorption wavelength of 259.5 nm was measured at 0，1，2，4，8，24 and 48 h，respectively.The total flavonoid contents were calculated，and the RSD was 1.0%within 8 h and 3.4%within 48 h.It indicated that the test solution was stable within 8 h，but unstable within 48 h.Therefore，the test solution could not be stood for a long time，and it is best to measure within 8 h.The results are shown in Table 12.