《Table 2 Pesticides and their dosage》

《Table 2 Pesticides and their dosage》   提示:宽带有限、当前游客访问压缩模式
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《Pesticides Could Alleviate the Symptoms of Sweet Potato Virus Disease(SPVD)》


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Viral real-time fluorescence quantitative primers[12]were synthesized by Shanghai Sangon Biotech Co.,Ltd.(Table 3).Realtime fluorescence quantitative PCR detection of c DNA of samples treated by the pesticides was performed using Toyobo SYBRGreen Realtime PCR Master Mix QPK201.The instrument used was ABI Stepone plus.The reaction process was as follows:it was conducted for 10 min at 95℃,15 s at 95℃,15 s at 60℃,and 20 s at 72℃,and there were 40 cycles.Determination of primer melting curves was conducted at 60-95℃.The gene of sweet potato Ib ARF(ADP-ribosylation factor)[13]was used as an internal control,the relative content of virus was calculated using the 2-ΔΔCtmethod(Table 3).