《Table 2 Pesticides and their dosage》
本系列图表出处文件名:随高清版一同展现
《Pesticides Could Alleviate the Symptoms of Sweet Potato Virus Disease(SPVD)》
Viral real-time fluorescence quantitative primers[12]were synthesized by Shanghai Sangon Biotech Co.,Ltd.(Table 3).Realtime fluorescence quantitative PCR detection of c DNA of samples treated by the pesticides was performed using Toyobo SYBRGreen Realtime PCR Master Mix QPK201.The instrument used was ABI Stepone plus.The reaction process was as follows:it was conducted for 10 min at 95℃,15 s at 95℃,15 s at 60℃,and 20 s at 72℃,and there were 40 cycles.Determination of primer melting curves was conducted at 60-95℃.The gene of sweet potato Ib ARF(ADP-ribosylation factor)[13]was used as an internal control,the relative content of virus was calculated using the 2-ΔΔCtmethod(Table 3).
图表编号 | XD00183149700 严禁用于非法目的 |
---|---|
绘制时间 | 2018.10.01 |
作者 | Hao XIE、Zaixing SU、Hui YAN、Qiang LI、Yaju LIU、Meng KOU、Wei TANG、Xin WANG、Yungang ZHANG |
绘制单位 | Sweet Potato Research Institute,Chinese Academy of Agricultural Sciences、Sweet Potato Research Institute,Chinese Academy of Agricultural Sciences、Jiangsu Xuzhou Sweet Potato Research Center,Key Laboratory for Biology and Genetic Breeding of Sweet Potato,M |
更多格式 | 高清、无水印(增值服务) |