《Table 1 Difference significance of RNA absorbance of samples with and without liquid nitrogen grind

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《A Simple and High Quality Method for Isolation and Extraction of Total RNA of Pholiota adipose》

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In the experiment，we divided three bottles of P.adipose mycelium pellet culture medium into two groups，three samples in each group，one group adopted Trizol single-step method with liquid nitrogen grinding to extract RNA，the other group without liquid nitrogen grinding.Then，we measured OD260value of the RNA of these two groups of samples，and analyzed the significance of the difference（Table 1）.According to the data in Table 1，RNA OD260value of three samples with liquid nitrogen grinding was 10.08，9.021 and9.847，respectively，while that without liquid nitrogen grinding was 3.346，2.758 and 2.941，respectively，all were smaller than the former，indicating that through liquid nitrogen grinding，the extracted RNA content was high and the degradation was low，consistent with the results of comparative experiment.The P value（T-test）of the two groups with or without liquid nitrogen grinding was 0.000 4（lower than 0.01），indicating that the two groups of data had extremely significant differences.Therefore，the liquid nitrogen ground samples were very poor for extracting high quality total RNA of P.adipose products.