《Table 1 BLAST-N results of 16Sr DNA gene of isolates which diameter of clear circles≥10 mm and thei

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《Comparative Research on Facultative Anaerobic Cellulose Decomposing Bacteria Screened from Soil and Rumen Content and Diet of Dairy Cow》

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L，Bacterial strains isolated from rumen fluid；N，Bacterial strains isolated from rumen residue；S，Bacterial strains isolated from rations；T，Bacterial strains isolated from soil.

For 29 strains of bacteria，both the gram-negative（GN）bacteria with coccus cell morphology and grampositive（GP）bacteria with bacilli cell morphology were found.By observation，many of GN bacteria were opaque yellowish-white colony with wet，convex and rounded characteristic.In addition，majority of GP bacteria were white colony with dry，convex and rounded profiles.Of the 29 strains，only four strains were GN bacteria with no spore，three of the four strains were isolated from rumen fluid，and the rest were from soil.Other 25 strains were GP bacteria with spore.The growth status of the strains in aerobic condition was not different compared with anaerobic condition，therefore，these strains were considered as facultative anaerobic bacteria.The diameters of clear zone of cellulose decomposing microorganisms were between 7-15 mm，and L5 was the largest，reaching to15 mm.Table 1 showed the diameters of clear zone no less than 10 mm.