《Table 1 GenBank accession numbers of related mitochondrial 16S rDNA and nuclear 18S rDNA gene seque

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《"Versuriga anadyomene, a newly recorded scyphozoan jellyfi sh(Scyphozoa: Rhizostomae) in Chinese waters"》

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Bold means the nucleotide sequences obtained from the present study.

The PCR products were examined on 1%agarose gels，stained with Genecolour TM（Biotium，USA），and photographed with transmitted illumination.The PCR-amplifi ed DNA fragments were purifi ed using a DNA Gel Extraction Kit（Sangon，China）.The purifi ed PCR products were cloned using a pUCm-T vector kit（Sangon Biotech Co.，Shanghai，China），and then sequenced with an ABI 3730 automated DNA sequencer at Sangon Biotech Co.（Shanghai，China）.A BLAST search for the sequences was performed in the NCBI database to confi rm their identities.In addition，related mitochondrial 16S rDNA and nuclear 18S rDNA gene sequences for Scyphozoa were downloaded from NCBI and aligned with the new sequence（Table 1）.The mitochondrial16S rDNA and nuclear 18S rDNA gene sequence of Aurelia sp.1（KX827342 for 16S rDNA，EU276014for 18S rDNA）was used as an outgroup.Sequences were aligned with the program Clustal X.Maximum likelihood analysis of mitochondrial 16S rDNA and nuclear 18S rDNA data was performed based on the GTR+G+I model and TN93+G+I model，respectively，which were selected using the Akaike information criterion（Akaike，1992）.Bootstrap values were calculated using 1000 iterations.All phylogenetic analyses were performed using MEGA 5.0 software（Tamura et al.，2011）.