《Table 3–Correlations of Euclidean distance (ED) and simple matching distance (SM) with hybrid perfo

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《Phenotypic and molecular diversity-based prediction of heterosis in pearl millet(Pennisetum glaucum L.(R.) Br.)》

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The basic genetic material for this study comprised two sets of parental lines.The first set（hereafter，referred as Set I）comprised of 213 lines，which involved 98 maintainer parents（designated between 1984 and 2004 at ICRISAT，Patancheru，India）and 115 restorer parents（designated between 1985 and1995 at ICRISAT，Patancheru，India）.The second set（hereafter，referred as Set II）comprised of 166 hybrid parents，which comprised 88 maintainer parents and 78 restorer parents bred at ICRISAT，Patancheru，India since 2004.Genotyping data was generated using 38 SSRs for 213 lines in Set I[11]and its subset of 28 SSRs for 166 lines in Set II[12].Genotyping data of both the sets were analyzed using Darwin 5.0[13].The SSRs were highly polymorphic and 30 of them were distributed over all seven linkage groups in earlier studies[14–17]（Table 1）.A dissimilarity matrix was calculated for pairs of maintainer parents（B lines）×restorer parents（R lines）using simple matching[13].Twenty-two and 29 hybrid combinations were identified for sets I and II，respectively，based on genetic distance between B and R lines.Hybrid combinations were identified，considering that pairs with diverse pedigree parents were selected，and the genetic distances between B and R lines of pairs represented all levels（low，medium and high）of genetic distance.Genetic distance varied from 0.19 to0.90 between B and R lines of Set I and from 0.17 to 0.93 in Set II lines.Seed of these identified hybrid combinations was produced in summer season of 2008（for Set I）and 2009（for Set II）.Twenty-two hybrids（20 B×R and 2 R×R）were developed from Set I lines using 20 B lines and 23 R lines.Twenty-nine B×R hybrids were developed from Set II lines using 29 each of maintainer and restorer parents.