《Table 1General characteristics of the MAGs retrieved in the present study from Guaymas Basin hydrot

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《深古菌门的核心代谢功能和热环境起源》

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aHigh quality:completeness>90%，contamination<5%；medium quality:completeness>50%，contamination<10%；low quality:completeness<50%or contamination>10%.

A phylogenomic tree of the MAGs from the current study（16）and public database（44）was further constructed using 16 concatenated conserved ribosomal protein sequences（see Section 2.5and Fig.1(b）).A total of 54 MAGs were classified into 11 different subgroups（Table S1），whereas six MAGs（ex4484＿135，ex4484＿205，M10＿bin214，M10＿bin241，M8＿bin163，and JdFR-01）were categorized as unclassified due to the lack of 16S r RNAgene sequences.Similar to the phylogenetic analysis based on16S rRNA gene sequences，the subgroups Bathy-21 and Bathy-22are still located at the root of the phylogenomic tree，which suggests that these two linages may represent the ancient Bathyarchaeota types.Subgroups 1，3，5，6，8，15，and 17 have corresponding genomes and show similar topology with the 16SrRNA gene phylogenetic tree.Nevertheless，the phylogeny for subgroups 13 and 23 was not matched between the 16S r RNA-based and concatenated ribosomal protein-based phylogenomic analyses（Table S1）.Considering the higher resolution and stability of the phylogenetic analysis based on concatenated conserved ribosomal protein sequences compared with 16S rRNA genes，the phylogenomic tree was used for further analysis.