《Table 3 Assessment of homologous recombination (HR) efficiency of cells treated with CRISPR interfe

  提示：宽带有限、当前游客访问压缩模式

本系列图表出处文件名：随高清版一同展现

《"Inhibition of KU70 and KU80 by CRISPR interference,not NgAgo interference,increases the efficiency of homologous recombination in pig fetal fibroblasts"》

1. 获取 高清版本忘记账户？点击这里登录

1. 下载图表忘记账户？点击这里登录

1) The plasmid included GFP-deficient reporter plasmid and pMD18T-GFP donor plasmid.

A completely randomized design was adopted in three experiments.In Exp.1，for detecting the silencing effect of CRISPRi system or NgAgoi system on each gene，12wells were randomly divided into four groups.Each group with three repeats was co-transfected with dCas9 plasmids and gRNA，or NgAgo plasmid and gDNA corresponding to the target site of each gene（Tables 1 and 2）.In Exp.2，for assessment of HR efficiency induced by CRISPRi system or NgAgoi system，18 wells were randomly divided into six groups.Each group with three repeats was cotransfected with dCas9 plasmids，optimal gRNA and HDR-GFP reporter plasmid，or NgAgo plasmid，optimal gDNA and HDR-GFP reporter plasmid（Tables 3 and 4）.In Exp.3，for assessment of the changes of key factors in the NHEJ and HR pathways，nine wells were randomly divided into three groups on each system.Each group with three repeats was co-transfected with dCas9 plasmids and optimal gRNA，or NgAgo plasmid and optimal gDNA，targeting KU70 or Ku80 gene（Table 5）.