《Table 1 Taxonomical characteristics of strain LWYZ003》

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《Isolation of Secondary Metabolites with Antimicrobial Activities from Bacillus amyloliquefaciens LWYZ003》

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Strain LWYZ0003 was first cultured on LB medium from agar slants for 2 d，then the healthy colonies were generated and inoculated into 500-mL shake f lasks，each of which contained 100 mL of seed medium.After culturing under 180 r/min at 30°C for 3 d，300 mL seed medium was inoculated into 5-L fermenter that contained 3 L fermentation medium（7 d）which was composed of 50 g/L glucose，30 g/L soybean meal，3 g/L peptone，9 g/L NaCl，0.4 g/L KCl，0.48 g/L CaCl 2，0.2 g/L NaHCO 3.The fermentation broth of LWYZ003 was accomplished by repeating the procedure detailed earlier to get 12 L fermentation broth（Table 2）.