《Table 1List of plasmids, strains, relevant characteristics, and origins.》

《Table 1List of plasmids, strains, relevant characteristics, and origins.》   提示:宽带有限、当前游客访问压缩模式
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《一种可实现合成生物传感器现场部署的增材制造方法》


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To study this gene network’s dose response,starter cultures of E.coli with pDW065 were grown overnight in LB media supplemented with 50 lgámLà1kanamycin.The cultures were diluted to 0.2 OD600(i.e.,an optical density measured at 600 nm)and allowed to grow until reaching 0.6 OD600.The cultures were then diluted again,supplemented with 0–100 lmoláLà13O-C12(Sigma-Aldrich Corporation,USA),and maintained at the exponential phase at 37°C until fully induced(about 8 h).Cultures at 0.6OD600were diluted to 1:100 in filtered phosphate-buffered saline(PBS,Thermo Fisher Scientific Inc.,USA)and fluorescence was measured using an AccuriTMC6 flow cytometer(Becton,Dickinson and Company,USA).