《Table 1List of plasmids, strains, relevant characteristics, and origins.》
To study this gene network’s dose response,starter cultures of E.coli with pDW065 were grown overnight in LB media supplemented with 50 lgámLà1kanamycin.The cultures were diluted to 0.2 OD600(i.e.,an optical density measured at 600 nm)and allowed to grow until reaching 0.6 OD600.The cultures were then diluted again,supplemented with 0–100 lmoláLà13O-C12(Sigma-Aldrich Corporation,USA),and maintained at the exponential phase at 37°C until fully induced(about 8 h).Cultures at 0.6OD600were diluted to 1:100 in filtered phosphate-buffered saline(PBS,Thermo Fisher Scientific Inc.,USA)and fluorescence was measured using an AccuriTMC6 flow cytometer(Becton,Dickinson and Company,USA).
图表编号 | XD0061019700 严禁用于非法目的 |
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绘制时间 | 2019.02.01 |
作者 | Daniel Wolozny、John R.Lake、Paul G.Movizzo、Zhicheng Long、Warren C.Ruder |
绘制单位 | Department of Biological Systems Engineering,Virginia Polytechnic Institute and State University、Department of Bioengineering,University of Pittsburgh、Department of Bioengineering,University of Pittsburgh、Department of Bioengineering,University of Pittsbu |
更多格式 | 高清、无水印(增值服务) |