《Table 1 Function analysis for 53 TDFs in S.spontaneum leaves under drought stress》

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《干旱胁迫下割手密基因cDNA-SCoT差异表达分析（英文）》

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The up-regulated TDFs reclaimed and purified were cloned and sequenced，and then their sequences were analyzed and screened through comparing NCBI database.A total of 120 up-regulated TDFs sequences were successfully obtained，including 37 unknown TDFs，30 unknown functional TDFs，and 53known functional TDFs（Table 1）.They all had higher similarity with accessed genes in NCBI database than the others.The 53 known functional TDFs could be classified into 10 functional groups（Fig.5），which included associated-functional protein related genes（20.75%），metabolism related genes（13.21%），communication and signal transduction related genes（13.21%），transcription regulated factor related genes（13.21%），transport pathway related genes（11.32%），environmental interaction related genes（9.43%），energy metabolism related genes（7.55%），protein synthesis related genes（3.77%），defense related genes（3.77%），cell component biological synthesis related gene（3.77%）.In this study，bidirectional sugar transporter，sucrose transporter（SUT），tubulin alpha，drought-inducible protein，IAA-auxin-responsive protein，plasma-membrane H+-ATPase，fucose synthase and dehydrin（DHN），the important regulatory factors in plant anti-reverse physiology，were up-regulated expression in S.spontaneum leaves under drought stress，which indicated that S.spontaneum colud positively respond to drought stress and actively defense to preserve the plant normal physiological function.