《Table 3 Orthogonal test on in vitro proliferation response of sturgeon peripheral blood lymphocytes

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《Isolation of Sturgeon Peripheral Blood Lymphocytes and the Optimal Proliferation Response Conditions》

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Orthogonal experimental designPHA，Con A and LPS were selected as the mitogen for lymphocyte proliferation，respectively.Orthogonal tests were performed on culture time，culture temperature，initial cell concentration，fetal calf serum concentration and mitogen concentration adopting an L25（56）orthogonal test design table.The specific experiment design is shown in Table 1.