《Table 4 Effects of BA and NAA on induction of adventitious shoots of Betula microphylla Bunge var.p

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《Tissue Culture of Betula microphylla Bunge var. paludosa》

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Note:Different lowercase letters in the same column indicate significant differences at the 0.05 level.

The leafy stem sections and the new axillary buds（Fig.1 b）were placed in the re-differentiation culture process.After eight weeks，the data were analyzed.As shown in Table 3，under the condition of same hormone concentrations（BA 2.0 mg/L，NAA 0.1 mg/L），different types of medium had a significant effect on the multiplication coefficient of the shoots（P<0.001）.In the WPM medium，the multiplication coefficient was highest（11.5），After one subculture，adventitious buds in WPM medium grew in large clusters，the stems were hard（Fig.1 c），the degree of lignification was slightly higher，and white adventitious roots grew out at the base of some adventitious buds.In the MS medium，the multiplication coefficient of adventitious buds was 10.6.According to Tukey's multiple comparisons，there was no significant difference in the induction of shoots between MS and WPM medium（P=0.238）.However，they showed significant differences compared with LS，1/2MS and SH.The adventitious shoots induced by MS medium were very young，and were easily broken by tweezers.There were no calluses at the base of the adventitious shoots induced by the two kinds of medium.Among different types of medium，the induction of adventitious shoots was weakest in the LS medium，with multiplication coefficient of only 1.3.A large callus was formed at the base of each explant，accompanied by several hairy roots.Adventitious shoots were formed occasionally on the calluses.