《Table 4.COI sequence barcoding by comparison of lowest inter-specific p-distances and P (AB) .》

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《Geometric morphometric and molecular evidence suggest a new fruit fly species in Bactrocera(Zeugodacus) tau complex(Diptera: Tephritidae)》

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*Parentheses define total haplotypes in each taxon.**Indicate significant isolation between two closest clades.

Among the well documented species in this subgenus，the barcoding threshold is set from the lowest p-distance between B.tau sensu stricto and B.cucurbitae，which yield 0.033（Table 4）.According to the phylogenetic tree，the lowest interspecific p-distances of Gr.I and Gr.II were computed against B.tau form C.On one hand，the p-distance between Gr.I and B.tau form C is lower than the barcoding threshold，implying that Gr.I belongs to B.tau form C.On the other hand，the lowest p-distance between Gr.II and B.tau form C is greater than the barcoding threshold，suggesting that the genetic variation between them is considerable at the species level.Therefore，Gr.I and B.tau form C were merged and recalculated as a single species.