《Table 3–Infection types of PmLK906, Ml92145E8-9, and Pm4b in response to Bgt isolate E09 and 14 iso

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《Molecular mapping of a novel wheat powdery mildew resistance gene Ml92145E8-9 and its application in wheat breeding by marker-assisted selection》

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To facilitate the use of Ml92145E8-9 in wheat breeding，MAS combined with phenotypic selection was performed to select homozygous progeny carrying the target gene.Line 92145E8-9was crossed separately with Gaokang 1 and Liangxing 99.A total of 1605 F2plants from the cross Liangxing 99×92145E8-9 were screened with codominant markers linked to Ml92145E8-9（Xsdauk13 and Xsdauk682）and MlLX99（Xcfd73 and Xwmc441）.A total of 905 plants carried Ml92145E8-9 and MlLX99，including 94 homozygotes；311 F2lines of the 905 lines carried only Ml92145E8-9 and 302 of the 905 F2lines carried MlLX99.Marker-assisted selection combined with powdery mildew resistance evaluation and agronomic trait selection was conductedindifferentgenerationsofLiangxing99×92145E8-9 and Gaokang 1×92145E8-9.F5:6progeny derived from Liangxing 99×92145E8-9 and F5:6progeny derived from Gaokang 1×92145E8-9 were tested with Bgt isolate E09 under controlled incubator conditions and separately with a natural Bgt population in the greenhouse（Table S2）.Three of the progeny of Gaokang 1×92145E8-9，named SDAU4173，SDAU4174，and SDAU4175，were screened with Xsdauk13 and Xsdauk682 to identify homozygous lines carrying the target gene Ml92145E8-9（Fig.S2-a，c）.The three progeny lines showed complete resistance to Bgt isolate E09，indicating that Ml92145E8-9 had been successfully transferred into SDAU4173，SDAU4174，and SDAU4175.SDAU3561 and SDAU3562，derived from Liangxing 99×92145E8-9，were screened with the markers closely linked to Ml92145E8-9（Xsdauk13 and Xsdauk682）and MlLX99（Xcfd73 and Xwmc441）.Both Ml92145E8-9 and MlLX99 had been successfully pyramided in lines SDAU3561 and SDAU3562，which showed complete resistance to Bgt isolate E09（Figs.S2-b，d，S3-a，b；Table S2）.Furthermore，the agronomic traits seed protein，plant architecture，and 1000-grain weight in the homozygous progenies were improved in comparison with 92145E8-9（Table S2）.It is noteworthy that the plant heights of SDAU4173，SDAU4174，SDAU4175，SDAU4174，and SDAU4175were lower than that of 92145E8-9，a finding promising for lodging resistance.