《Table 1–The types of starch in the endosperm of common millet.》

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《Waxy allelic diversity in common millet(Panicum miliaceum L.) in China》

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PCR amplification was performed with three sets of primers（Table S2）synthesized by BGI Tech Solutions Co.，Ltd.（Beijing，China）.Primer sets int5Lf-R3 and M12-R12 were used to amplify the intron 5–exon 7 and intron 8–9 sequences，respectively，of the Wx-L gene[23，26].Primer set M5-R11 was used to amplify the exon 9–intron 10 sequence of the Wx-S gene.PCR amplification was performed in a solution of 25μL total volume containing 2.5μL 10×PCR buffer（ZOMANBIO，Beijing，China），0.5μL of each primer（0.5μmol L-1），2μL d NTP（200μmol L-1），0.5μL DNA polymerase（1.3 U），16μL dd H2O，2.5μL 10×Taq buffer，and 3μL DNA template.M5-R11 and M12-R12 were amplified using the following temperaturecycling parameters:94°C for 2 min，followed by 30 cycles of94°C for 30 s，54°C for 30 s，and 72°C for 1 min 30 s，with a final extension at 72°C for 7 min.The Int5Lf-R3 region was amplified at 94°C for 2 min，followed by 35 cycles of 94°C for45 s，54°C for 30 s，and 72°C for 2 min，followed by a final extension at 72°C for 7 min.PCR amplicons with single discrete bands from agarose gel electrophoresis were purified.