《Table 4 Prevalence of IL-17-producing Treg cells in PE (N=23) and in NP (N=40)》

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《The altered PD-1/PD-L1 pathway delivers the ‘one-two punch’ effects to promote the Treg/Th17 imbalance in pre-eclampsia》

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Abbreviations:NP，normal pregnancy；PE，pre-eclampsia.Data are presented as the mean±s.e.m.

This study was reviewed and approved by Huazhong University of Science and Technology Clinical Trial Ethics Committee.All methods were carried out in accordance with the approved guidelines and regulations.Written informed consent was obtained from each participant prior to entering the study.All the study subjects，namely，healthy pregnant women and pre-eclamptic women，were recruited from the Department of Obstetrics and Gynecology at the Maternal and Child Health Hospital of Hubei Province，Wuhan，China.The presence of PE was assessed according to ACOG guidelines（ACOG Task Force on Hypertension in Pregnancy，2013）.All pregnancies in this study were singleton gestations，and none of the participants had active labor at the time of enrollment and blood sampling.None of the participants was affected by preexisting clinical disorders，such as renal diseases or chronic hypertension before pregnancy，or complicated by pre-term labor，chorioamnionitis or diabetes.Normal pregnant women in the third trimester were matched with the pre-eclamptic women for age，gestational age and parity（Table 1）.