《Table 6Chemical ingredient of methanolic leaf extract fraction of C.crista by LC-MS.》

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《Phytochemical composition of Caesalpinia crista extract as potential source for inhibiting cholinesterase and β-amyloid aggregation: Significance to Alzheimer's disease》

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The TLC of CCMe OH extract showed different bands（Figure 5），which shows the presence of various phytochemical presence.For further study of these phytochemials，we performed GC-MS and LC-MS.GC-MS analysis of CCMeOH extract was carried out.Peaks obtained were shown in chromatogram（Figure 6）and a total of 26compounds were identified with their peak number，retention time（RT），area under peak（%）and height of the peak（%）as represented in the Table 4.Most of the identified compounds have medicinal properties.For LC-MS study，the column chromatography was carried out with 60-120 mesh silica.Total of seventeen fractions were collected，among which only sixteenth and seventeenth were brown compared to rest that was yellow or green in color，and were eliminated and subjected to LC-MS along with whole CCMeOH extract.The whole extract of CCMeOH shown in the MS spectra（Figure 7Aa and 7Ab）demonstrated the various peaks represented the presence of phytochemicals that was considered with retention time 3.124（7Aa）and 5.839（7Ab）of LC chromatogram.The column fraction of CCMe OH was brown color fraction as in Figure 7B and the MS spectra showed retention time 3.124（7Ba）and 3.517（7Bb）of LC chromatogram.Both the spectra mass was compared with help of MSLAMPS database.In Table 5，we could be able to identify thirteen polyketides and one prenol lipids as in Figure 7A showing the MS spectra of RT time 3.124（7Aa）and 5.839（7Ab）of LC chromatogram for CCMe OH whole extract.Both the spectra mass was also compared with help of MSLAMPS database.As in Table 6，we could be able to identify nine polyketides for CCMe OH column fraction from MS chromatogram 7Ba and 7Bb.Some of the mass peak in Figure 7B with RT 3.124（7Ba）MS spectra of CCMeOH column fraction was as same as in CCMe OH whole extract in Figure7Aa with RT 3.124（7Aa）which was excluded.Both the whole fraction and column fraction molecules compared with mass number represented indicate the presence of flavanoids like kaempferol，myricitin，catechin，quercetin，chrysosplenol etc.Spectral studies give an insight into the presence of active ingredients present in the extracts which could be isolated，and the studies in detail show the plausible mechanism that may play a vital role in inhibition of pathogenesis of AD.