《Table 1 Treatment with CRISPR interference (CRISPRi) system (Exp.1)》
本系列图表出处文件名:随高清版一同展现
《"Inhibition of KU70 and KU80 by CRISPR interference,not NgAgo interference,increases the efficiency of homologous recombination in pig fetal fibroblasts"》
A completely randomized design was adopted in three experiments.In Exp.1,for detecting the silencing effect of CRISPRi system or NgAgoi system on each gene,12wells were randomly divided into four groups.Each group with three repeats was co-transfected with dCas9 plasmids and gRNA,or NgAgo plasmid and gDNA corresponding to the target site of each gene(Tables 1 and 2).In Exp.2,for assessment of HR efficiency induced by CRISPRi system or NgAgoi system,18 wells were randomly divided into six groups.Each group with three repeats was cotransfected with dCas9 plasmids,optimal gRNA and HDR-GFP reporter plasmid,or NgAgo plasmid,optimal gDNA and HDR-GFP reporter plasmid(Tables 3 and 4).In Exp.3,for assessment of the changes of key factors in the NHEJ and HR pathways,nine wells were randomly divided into three groups on each system.Each group with three repeats was co-transfected with dCas9 plasmids and optimal gRNA,or NgAgo plasmid and optimal gDNA,targeting KU70 or Ku80 gene(Table 5).
图表编号 | XD0047433900 严禁用于非法目的 |
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绘制时间 | 2019.02.20 |
作者 | LI Guo-ling、QUAN Rong、WANG Hao-qiang、RUAN Xiao-fang、MO Jian-xin、ZHONG Cui-li、YANG Hua-qiang、LI Zi-cong、GU Ting、LIU De-wu、WU Zhen-fang、CAI Geng-yuan、ZHANG Xian-wei |
绘制单位 | National Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural University、National Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural Universi |
更多格式 | 高清、无水印(增值服务) |