《Table 2.Matrix of genetic distances (FST) among populations of Enhalus acoroides calculated by usin

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《"Assessment by microsatellite analysis of genetic diversity and population structure of Enhalus acoroides from the coast of Khanh Hoa Province, Vietnam"》

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Note:*Significant different atα=0.05，p-value<0.01.See legend in Fig.1 for the abbreviation of the locations.

The related low genetic difference was detected among populations with an overall FST value of 0.25.The pairwise genetic differentiation depicted that the highest genetic differentiation was found between MG and SL（FST=0.47，p-value<0.01）whereas these values were lowest among populations growing in lagoon including the TT，CPN and CT sites（pairwise FST<0.06，pvalue>0.1）（Table 2） .The results of structure analysis indicated that six populations formed two clusters，one including the open sea populations（TL，MG and SL:open sea cluster 1）and one including the lagoon populations（TT，CPN and CT:lagoon cluster2）（Fig.2，Panel A） by both methods.The largest variation of value L（K）was observed at K=2 according to the method of Rosenberg et al.（2001）and the highestΔK was also found at K=2according to the method of Evanno et al.（2005）（data not shown） .The cluster analysis also showed that six populations separated into two main clades including open sea（TL，MG and SL）and lagoon（TT，CPN and CT）（Fig.2，Panel B） .The results of AMOVA showed 9.5%（p-value<0.01）of variation between the two clusters.The percentages of variation among populations within populations were 15.6（p-value<0.01）and 74.2%（pvalue>0.1），respectively（Table 3）.The isolation-by-distance parameter resulting from pairwise plotting of FST values against the geographic distance（km）was generated using the Mantel test for all regions.The Mantel test showed no significant isolation by distance at a significance level of 95%.The mantel r statistic of 0.41 indicates that there is a relatively low positive correlation between genetic and geographic distance.However，these results are not statistically significant atα=0.05（Fig.3）.