《Table 1 NMR and GPC characterizations ofα/βCPMBs》

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《具有高效抗MRSA活性的Alpha-Beta杂化多肽聚合物分子刷（英文）》

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a) GPC characterization on polypeptide at the amine protected stage using DMF as the mobile phase at a flow rate of 1 mL min-1.

The backbone of theα/βCPMB，aβ-polypeptide or poly-β-amino acid（PβAA），was synthesized from a base catalyzed anionic ROP of 1:1 mixture of twoβ-lactams by following a previously reported method，with oneβ-lactam having a hydrophobic sidechain and the otherβ-lactam having an amine-containing sidechain[25，66].The pendent amine groups of theβ-polypeptide backbone then serve as activation sites for the ROP ofα-L-Lys-NCA to provide theα/βCPMB as described in Fig.1.Theβ-polypeptide backbone was synthesized with narrow polydispersity index（PDI）of 1.20 as summarized in Table 1.The average degree of polymerization（DP）of thisβ-polypeptide backbone was found to be 18 using gel permeation chromatography（GPC）characterization.In order to further attach poly-α-L-lysine onto to theβ-polypeptide backbone and generate theα/βCPMBs，we choose the extensively used NCA polymerization.By using the backbone pendent amine groups as the initiation points，the poly-α-L-lysine chains were grafted from the backbone.Twoα/βCPMBs，PβAA-g-PαLL10and PβAA-g-PαLL20，were synthesized to have different length of sidechain poly-α-L-lysine，with DP designed to be 10and 20 respectively.The GPC characterization clearly indicated increase of Mnfrom theβ-polypeptide backbone（PβAA）at 3.3 kDa to the finalα/βCPMBs（PβAA-g-PαLL10and PβAA-g-PαLL20）at about 33.4 and 48.1 kDa and a narrow PDI at 1.28 and 1.24，respectively.DP of theβ-polypeptide backbone and finalα/βCPMBs were also confirmed using nuclear magnetic resonance（NMR）spectra and the results were comparable to those obtained from GPC characterization.The diameters ofα/βCPMBs were measured by dynamic light scattering（DLS）to get an average particle size of 8.35±2.02 nm for PβAA-gPαLL10and 17.22±4.14 nm for PβAA-g-PαLL20in a solution of phosphate buffered saline（PBS）at 0.5 mg mL-1of polymer（Fig.S1）.