《Table 3 Maximum allowable deviation of relative ion abundance during qualitative detection》

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《Establishment and Optimization of HPLC-MS/MS for Detection of Progesterone Residue in Raw Milk》

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The LC separation used an Eclipse Plus C18column（2.1 mm×100 mm，1.8μm）with A（5 mmol/L ammonium acetate solution+0.1%formic acid）and B（acetonitrile）as the mobile phase for gradient elution（the gradient elution conditions are shown in Table 1）at a flow rate of 0.3 ml/min under a column temperature of 40℃.Under these conditions，progesterone showed good retention with a retention time of about 2.70 min（Fig.1）.