《Table 2 Contamination rate of C.goeringii new buds with different disinfection treatments》

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《国兰组培中外植体高效消毒方法研究（英文）》

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As shown in Table 2，when the new buds were treated with 0.1%mercuric chloride for 3，5，and 8min，the contamination rates were 40%，25%，and15%，the death rates were 40%，45%，and 65%，and the number of new buds with callus were 2，3，and1 respectively.In this case，the disinfection effects of 0.1%mercuric chloride for new buds increased with the change of the contact time，although they were still inferior to those in the seed experiments.The maximal contamination rate was 40%，and the minimal contamination rate was 15%.However，the death rate of new buds increased drastically with the extension of the disinfection time；the peak was a65%.One possible cause might be the strong toxicity of mercuric chloride.Excessive disinfection time or improper flushing could result in the death of the explants.When the buds were disinfected with 10%sodium hypochlorite for 10，15，and 20 min，the contamination rates were 55%，30%，and 25%，and the death rates were 60%，45%，and 60%respectively.The disinfection trend of 10%sodium hypochlorite for new buds was similar to that of 0.1%mercuric chloride the extension of the disinfection time could lead to a lower contamination rate，but a higher death rate.In general，the growth status of the callus tissue was no good under all treatments，presenting a low callus rate possibly because of the improper concentrations of growth hormone and cytokinin，as well as inadequate induction time.To conclude，75%ethanol for 45 s+0.1%mercuric chloride for 5 min provided the optima disinfection effects in the bud experiments.