《An Introduction To Paper Electrophoresis and Related Methods》

1．Introduction1

Historical1

Tiselius method2

Scope of the Tiselius method5

Terminology7

References8

2．Electrical Conduction of Solutions10

Essentials of electrochemistry10

Temperature variation of conductivity11

Variation of conductivity with dilution12

Independent mobility and absolute velocity of ions14

3．Theoretical Considerations17

Factors influencing the movement of particles in an electric field17

Theory of paper electrophoresis23

References30

4．Technique of Paper Electrophoresis31

Methods of supporting the filter paper31

Other technical aspects44

Preparative methods46

References49

5．Continuous Electrophoretic Separation50

Principle50

Apparatus52

Some applications57

References60

6．Electrophoresis on the Cytoscale61

7．Combination of Chromatography and Electrophoresis63

Simultaneous chromatography and electrophoresis63

Electrophoresis followed by chromatography64

References66

8．Organic Acids67

Higher fatty acids67

Keto acids68

Di-and Tricarboxylic acids68

References69

9．Carbohydrates and Hydroxycompounds70

Monosaccharides71

Methyl ethers and disaccharides73

Polysaccharides75

Polyhydric alcohols and phenols77

Flavonoids79

References80

10．Nitrogen Compounds81

Amines81

Aminosugars81

Indole derivatives81

Sulphanilamides82

Amino acids and peptides83

Two-dimensional separation of amino acids86

DNP-amino acids90

Guanido derivatives89

Peptides90

Iodothyronines and iodotyrosines90

References91

11．Alkaloids92

12．Proteins95

Details of the electrophoretic techniques95

Quantitative evaluation of proteins on the paper98

Detection of lipoids in serum fractions on the paper106

Estimation of fibrinogen108

Lysozyme109

The subfractions of the serum globulins109

Some clinical results112

References120

13．Nucleic Acids and Derivatives,and Phosphate Esters125

Nucleic acids125

Hydrolysis products of nucleic acids126

Separation of ATP,ADP and related substances130

Thiamine and thiamine phosphoric esters132

Vitamin B2 and B6 groups134

References135

14．Antibiotics136

15．Triterpenoids138

16．Steroids139

17．Dyes141

18．Inorganic Compounds143

Sequences of ions143

Separations of analytical and preparative interest147

References155

19．Physico-chemical Studies156

20．Electrophoresis in Packed Columns162

Electrophoresis in a glass powder column163

Electrophoresis in starch columns164

Electrophoresis in a cellulose column165

Electrophoresis in slabs of cellulose or starch166

References168

21．Electrophoresis Inside Gels169

Electrophoresis in silica jelly169

Electrophoresis in agar gel174

References186

22．Measurement of Diffusion Rates Inside Porous Media187

23．Electrokinetic Ultrafiltration189

Author Index193

Subject Index199