《AN INTRODUCTION TO PAPER ELECTROPHORESIS AND RELATED METHODS》

1．Introduction1

Historical1

Tiselius method2

Scope of the Tiselius method5

Terminology7

References8

2．Electrical Conduction of Solutions10

Essentials of electrochemistry10

Temperature variation of conductivity11

Variation of conductivity with dilution12

Independent mobility and absolute velocity of ions14

3．Theoretical Considerations17

Factors influencing the movement of particles in an electric field17

Theory of paper electrophoresis23

References30

4．Technique of Paper Electrophoresis31

Methods of supporting the filter paper31

High voltage paper electrophoresis44

Separation with a potential gradient48

Radial development48

Other technical aspects49

Preparative methods51

References53

5．Continuous Electrophoretic Separation55

Principle55

Apparatus57

Some applications62

References65

6．Electrophoresis on the Cytoscale66

7．Combination of Chromatography and Electrophoresis68

Simultaneous chromatography and electrophoresis68

Electrophoresis followed by chromatography69

References71

8．Organic Acids72

Higher fatty acids72

Keto acids73

Di-and Tricarboxylic acids73

References74

9．Carbohydrates and Hydroxycompounds75

Monosaccharides76

Methyl ethers and disaccharides78

Polysaccharides80

Polyhydric alcohols and phenols82

Flavonoids84

References85

10．Nitrogen Compounds86

Amines86

Aminosugars86

Indole derivatives86

Sulphanilamides87

Amino acids and peptides88

Two-dimensional separation of amino acids91

DNP-amino acids94

Guanido derivatives94

Peptides94

Iodothyronines and iodotyrosines95

References96

11．Alkaloids97

12．Proteins100

Details of the electrophoretic techniques100

Quantitative evaluation of proteins on the paper103

Detection of lipoids in serum fractions on the paper111

Estimation of fibrinogen113

Lysozyme114

The subfractions of the serum globulins114

Some clinical results117

References126

13．Nucleic Acids and Derivatives,and Phosphate Esters131

Nucleic acids131

Hydrolysis products of nucleic acids132

Separation of ATP,ADP and related substances136

Thiamine and thiamine phosphoric esters138

Vitamin B2 and B6 groups140

References141

14．Antibiotics142

15．Triterpenoids144

16．Steroids145

17．Dyes147

18．Inorganic Compounds149

Sequences of ions149

Separations of analytical and preparative interest153

References161

19．Physico-chemical Studies163

20．Electrophoresis in Packed Columns169

Electrophoresis in a glass powder column170

Electrophoresis in starch columns171

Electrophoresis in a cellulose column172

Electrophoresis in slabs of cellu-lose or starch173

References175

21．Electrophoresis Inside Gels176

Electrophoresis in silica jelly176

Electrophoresis in agar gel181

References193

22．Measurement of Diffusion Rates Inside Porous Media194

23．Electrokinetic Ultrafiltration196

Author Index199

Subject Index206