《Table 3–Alpha diversity of soils in different treatments.》

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《Microbial response to CaCO_3 application in an acid soil in southern China》

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Results are expressed as mean value±standard error（n=4）.Different letters denote significant differences among treatments（p<0.05）.

Soil DOC and DON，as important parts of the nutrition cycling，are vital indices for evaluating soil fertility and characterizing soil productivity，and have essential influence on soil microbial activity and crop growth（Li et al.，2012；Six et al.，2002）.Our results showed that the contents of DOC and DON in soil were significantly increased by moderate dose of CaCO3application（Table 1），indicating that proper application of CaCO3could significantly improve soil nutrition cycling.This result was well consistent with that of Kreutzer（1995），who found CaCO3application can modify soil organic matter composition by enhancing DOC contents，C turnover and humus storage，all of which were important processes of soil functions.The increasing of DOC and DON induced by CaCO3application could be attributed to the mitigation of soil acidification and activation of soil microbes.It has been demonstrated that under neutral pH conditions，the solubility of organic matter could be significantly increased，and activity of microbes involved in decomposition of soil organic matter could be enhanced（L?fgren et al.，2009；Paradelo et al.，2015）Rangelcastro et al.（2005）also demonstrated that turnover of C and N could be more rapid in the limed soil due to higher microbial activities.However，under HC treatment，DOC and DON contents decreased compared to control（Table 1）Excessive CaCO3application could lead to the inadaptability of microbes，in addition，some DOC can be easily neutralized with other substances such as calcium and magnesium compounds under high pH conditions，resulting in decline of DOC content.