《Table 3 Effect of pH on the activity of antimicrobial substances》

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《一株新型稻瘟霉素链霉菌JZB130180的鉴定及其对桃褐腐病的生防效果评价（英文）》

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The classification was confirmed by sequencing of the 16S ribosomal DNA（rDNA），atpD，and recA genes.S.blastmyceticus JZB130180 was cultured in potato dextrose（PD）broth in a rotary shaker for 48 h at 28°C and 180 r/min.The supernatant was removed by centrifugation，pelleted cells were collected，and genomic DNA was extracted by cetyltrimethyl ammonium bromide（CTAB）method（Wang et al.，2011）.Using the genomic DNA of S.blastmyceticus JZB130180 as the template，the 16S rDNA，atpD，and recA sequences were amplified by polymerase chain reaction（PCR）using corresponding primers（Table S1）（Weisburg et al.，1991；Guo and Huang，2007） .The resulting sequences were compared with those of known strains using the National Center for Biotechnology Information（NCBI）BLAST search（http://www.ncbi.nlm.nih.gov/BLAST）.A phylogenetic tree based on the concatenated 16S rDNA，atpD，and recA sequences was constructed（Vaidya et al.，2011）using the maximum parsimony（MP）method in PAUP*4.0b10，and the support of branching topologies was derived from 1000 replicates with 10 random additions（Wilgenbusch and Swofford，2003）.